Association of the rs12617656 C/T genetic variant of the DPP4 gene with in-stent restenosis in Mexican population: a cohort study

Gilberto Vargas-Alarcón, Departamento de Biología Molecular, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, México
Rosalinda Posadas-Sánchez, Departamento de Endocrinología, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, México
Marco A. Martínez-Ríos, Departamento de Hemodinámica, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, México
Hilda Delgadillo-Rodriguez, Departmento de Consulta Externa. Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, México
Victoria López-Olmos, Departamento de Biología Molecular, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, México
José M. Fragoso, Departamento de Biología Molecular, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, México

Objective: We evaluated whether five (rs12617336, rs12617656, rs1558957, rs3788979, and rs17574) single nucleotide polymorphisms located in the DPP4 gene that have not been sufficiently explored, are candidates to be risk markers of in-stent restenosis. Methods: The genotypes were determined in 190 patients (60 patients with restenosis and 130 without restenosis) using 5’exonuclease TaqMan assays in accordance with the manufacturer’s instructions (Applied Biosystems, Foster City, USA). Results: The results showed that the CC genotype of the rs12617656 C/T SNP was associated with the risk of development restenosis after coronary stent under the co-dominant, and recessive models (odds ratios [OR] = 3.32, p = 0.0009, and OR = 4.96, p = 0.0008, respectively). In addition, our data showed that the genetic distribution of the rs12617336, rs1558957, rs3788979, and rs17574 SNPs were similar between patients with and without restenosis after coronary stenting. On the other hand, the haplotype analysis showed one haplotype (CTC) associated with restenosis susceptibility (p = 0.006). Conclusion: Our study demonstrates that the rs12617656 genetic variant of the DPP4 gene is associated with the risk of developing in-stent restenosis in our population.

Keywords: Association. Single nucleotide polymorphisms. In-stent restenosis.